Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Article Title: Locus Coeruleus-Dorsolateral Septum Projections Modulate Depression-Like Behaviors via BDNF But Not Norepinephrine.

doi: 10.1002/advs.202303503

Figure Lengend Snippet: Figure 7. BDNF was necessary for the LCTH-dLS circuit to sustain the antidepressant-like effects of S-ketamine but not fluoxetine. A) Schematic rep- resentation of the Cre-dependent AAV expressing BDNF-shRNA-EGFP in the LCTH-dLS circuit. B) Timeline of experiments. C) Representative social interaction tracks of BDNF-shRNA and Con-shRNA mice and the time spent in the interaction zone in the SIT in different groups (n = 9). D) Locomotor activity and depression-like behaviors in BDNF-shRNA and control-shRNA mice injected with saline or S-ketamine (n = 9). E) Schematic representation of virus injection. F) Timeline of the experiments. G) Representative social interaction tracks of BDNF-shRNA and Con-shRNA mice and the time spent in the interaction zone in the SIT in different groups (n = 8). H) Locomotor activity and depression-like behaviors in BDNF-shRNA and Con-shRNA mice injected with saline or fluoxetine (n = 8). Data represented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001; n.s., not significant. Two-way ANOVA followed by Bonferroni’s post hoc analysis for (C,D) and (G,H). The statistical details can be found in Table S1, Supporting Information.

Article Snippet: Drugs were prepared: CNO (1 mg kg−1, i.p., diluted in 0.9% saline; 3 mM, 1 μL per site, CAS NO.: C0832; Sigma-Aldrich), K252a (1 μM, 1 μL per site, CAS NO.:99533-80-9, Sigma-Aldrich), propranolol (5 mM, 1 μL per site, CAS NO.:318-98-9, Sigma-Aldrich), phentolamine (10 mM, 1 μL per site, CAS NO.: 73-05-2, Sigma-Aldrich), Recombinant Human BDNF Protein (200 ng in 0.9% saline for local injection, 1 μL per site, CAS NO.:248-BDB; R&D Systems), anti-BDNF neutralizing antibody (0.2 μg in 0.9% saline for local injection, 0.2 μL per site, EMD Millipore), Sketamine (10 mg kg−1, i.p., diluted in 0.9% saline, CAS NO.:1430202-702, Sigma-Aldrich), R-ketamine (10 mg kg−1, i.p., diluted in 0.9% saline, CAS NO.: 1430202-69-9, Sigma-Aldrich), and fluoxetine (20 mg kg−1 i.p., diluted in 0.9% saline, CAS NO.:56296-78-7, Sigma-Aldrich).

Techniques: Expressing, shRNA, Activity Assay, Control, Injection, Saline, Virus

Journal: Experimental gerontology

Article Title: Walking Speed Decline in Older Adults is Associated with Elevated Pro-BDNF in Plasma Extracellular Vesicles

doi: 10.1016/j.exger.2017.08.024

Figure Lengend Snippet: Recombinant BDNF was added in concentrations of 62-4000 pg/dl to proBDNF ELISA plates (A) and recombinant proBDNF was added in concentrations of 62 – 4000 pg to BDNF ELISA plates (B). The signal produced was compared to the signal produced by the same concentration of recombinant proBDNF (A) or recombinant BDNF (B). Each curve represents a separate experiment on a separate ELISA plate, while the samples were run in duplicates. C. On a proBDNF ELISA plate, increasing concentrations of recombinant BDNF were added to 3.3 or 25 ng of recombinant proBDNF and the measured concentration of proBDNF was calculated based on the standard curve produced by pure recombinant proBDNF. The fact that the line remains almost horizontal at the excpected concentration shows the reproducibility of the ELISA and its validity when measuring mixtures of BDNF and proBDNF. D. On a BDNF ELISA plate, increasing concentrations of recombinant proBDNF were added to 500 or 1000pg of recombinant BDNF and the measured concentration of BDNF was calculated based on the standard curve produced by pure recombinant BDNF. The fact that the line remains almost horizontal at the excpected concentration shows the reproducibility of the ELISA and its validity when measuring mixtures of BDNF and proBDNF.

Article Snippet: Enzyme-linked immunosorbent assays (ELISAs) Mature BDNF (BDNF Emax ® ImmunoAssay System, Promega, cat. #G7611) and proBDNF (Pro BDNF [Human, Mouse, Rat] ELISA Kit, Aviscera Bioscience, cat#s SK00752-08 and SK00752-09) ELISAs were performed on aliquots of plasma, and total EVs or L1CAM+ EVs isolated from the plasma, using the protocols of the assay manufacturer's.

Techniques: Recombinant, Enzyme-linked Immunosorbent Assay, Produced, Concentration Assay

Journal: Experimental gerontology

Article Title: Walking Speed Decline in Older Adults is Associated with Elevated Pro-BDNF in Plasma Extracellular Vesicles

doi: 10.1016/j.exger.2017.08.024

Figure Lengend Snippet: A. BDNF concentrations in L1CAM+ EVs, plasma, and total EVs, in a preliminary cohort of 20 participants showing higher levels of BDNF within L1CAM+ EVs. B. ProBDNF concentrations in L1CAM+ EVs, plasma, and total EVs, in a preliminary cohort of 20 participants showing higher levels of BDNF within L1CAM+ EVs.

Article Snippet: Enzyme-linked immunosorbent assays (ELISAs) Mature BDNF (BDNF Emax ® ImmunoAssay System, Promega, cat. #G7611) and proBDNF (Pro BDNF [Human, Mouse, Rat] ELISA Kit, Aviscera Bioscience, cat#s SK00752-08 and SK00752-09) ELISAs were performed on aliquots of plasma, and total EVs or L1CAM+ EVs isolated from the plasma, using the protocols of the assay manufacturer's.

Techniques: Clinical Proteomics

Journal: Experimental gerontology

Article Title: Walking Speed Decline in Older Adults is Associated with Elevated Pro-BDNF in Plasma Extracellular Vesicles

doi: 10.1016/j.exger.2017.08.024

Figure Lengend Snippet: A. Adjusted odds ratio (OR) for time 1, where only proBDNF L1CAM+ EVs predict decliners. Models adjusted for age, sex, plate number, percent fat mass, baseline gait speed, and comorbidity index. B. Adjusted OR for time 2, where only proBDNF L1CAM+ EVs predict decliners. Models adjusted for age, sex, plate number, percent fat mass, baseline gait speed, and comorbidity index.

Article Snippet: Enzyme-linked immunosorbent assays (ELISAs) Mature BDNF (BDNF Emax ® ImmunoAssay System, Promega, cat. #G7611) and proBDNF (Pro BDNF [Human, Mouse, Rat] ELISA Kit, Aviscera Bioscience, cat#s SK00752-08 and SK00752-09) ELISAs were performed on aliquots of plasma, and total EVs or L1CAM+ EVs isolated from the plasma, using the protocols of the assay manufacturer's.

Techniques: Clinical Proteomics

Journal: Experimental gerontology

Article Title: Walking Speed Decline in Older Adults is Associated with Elevated Pro-BDNF in Plasma Extracellular Vesicles

doi: 10.1016/j.exger.2017.08.024

Figure Lengend Snippet: A. BDNF readings from L1CAM+ EVs are not significantly different between decliners and non-decliners or visits. B. proBDNF readings from L1CAM+ EVs are significantly different between decliners and non-decliners at both time points. C. The ratio of BDNF to proBDNF in L1CAM+ EVs is not significantly different between decliners and non-decliners or time points.

Article Snippet: Enzyme-linked immunosorbent assays (ELISAs) Mature BDNF (BDNF Emax ® ImmunoAssay System, Promega, cat. #G7611) and proBDNF (Pro BDNF [Human, Mouse, Rat] ELISA Kit, Aviscera Bioscience, cat#s SK00752-08 and SK00752-09) ELISAs were performed on aliquots of plasma, and total EVs or L1CAM+ EVs isolated from the plasma, using the protocols of the assay manufacturer's.

Techniques: